mouse muc2 specific antibody  (Novus Biologicals)

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet: (A–E) Representative cross-sections of distal mouse colon fixed in Carnoy’s solution at (A) day 1 (D1), (B) D2, (C) D4, (D) D7, and (E) D42 of colonization by Bt . Blue, DAPI staining in the epithelium; red, DAPI staining in the lumen of gut, including bacteria, debris, and shed host nuclei; green, antibody staining for MUC2. Scale bars, 10 μm. (F) Mean Bt epithelial proximity. n = 3–4/group, t test, *p < 0.05.

Article Snippet: After deparaffinization and rehydration, slides were incubated in lysozyme solution at 37°C for 20 min, and then in antigen retrieval solution (10 mM sodium citrate [pH 6.0]) at 90°C for 10 min. For mucus visualization, a polyclonal rabbit anti-mouse Muc2-specific antibody (Santa Cruz Biotechnology) was diluted 1:100 in blocking buffer (Dako), applied to the slide, and incubated for 2 h in the dark at room temperature.

Techniques: Staining, Bacteria

Journal: Cell reports

Article Title: Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization

doi: 10.1016/j.celrep.2023.113009

Figure Lengend Snippet:

Article Snippet: After deparaffinization and rehydration, slides were incubated in lysozyme solution at 37°C for 20 min, and then in antigen retrieval solution (10 mM sodium citrate [pH 6.0]) at 90°C for 10 min. For mucus visualization, a polyclonal rabbit anti-mouse Muc2-specific antibody (Santa Cruz Biotechnology) was diluted 1:100 in blocking buffer (Dako), applied to the slide, and incubated for 2 h in the dark at room temperature.

Techniques: Virus, Mutagenesis, Isolation, Recombinant, Saline, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, cDNA Synthesis, Shotgun Sequencing, Sequencing, Software, Spectrophotometry, Mass Spectrometry, Microscopy